Method Name
Platelet Aperature Occlusion
Aliases
Platelet Function Analysis; PFA; PFA-100; Bleeding Time, PFA-100
Specimen Required
Discard one blue top tube and then collect: (2) Blue top tubes and (1) Lavender top tube. Specimen should be collected with a needle large than 21g (i.e. 20g, 19g, 18g, etc.).
Specimen Minimum Volume
Blue top tubes must be filled
Specimen Stability Information
Specimens are stable for 4 hours at room temperature.
Unacceptable specimens include:
- Short samples (blue top tubes that are not filled +/- 10% fill volume.
- Specimen drawn with needles smaller than 21g
- Specimens drawn with butterfly needle.
- Specimens sent through the pneumatic tube system.
Special Instructions
This test replaces the Simplate Bleeding Time Test.
Performing Laboratory
As ordered
Reference Values
COL/EPI: 80 – 184 seconds COL/ADP: 56 – 102 seconds